{"product_id":"acridine-orange-propidium-iodide-stain-5-x-0-1ml","title":"Acridine Orange\/Propidium Iodide Stain, 5 X 0.1mL","description":"\u003cp\u003e\u003cstrong\u003eAcridine Orange\/Propidium Iodide Stain\u003cspan\u003e, 5 X 0.1mL\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e5 x 0.1 mL\u003c\/p\u003e\n\u003cp\u003eAcridine Orange\/Propidium Iodide Stain is a cell viability dye that causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. Acridine Orange\/Propidium Iodide Stain can be used to assess cell viability with the automated fluorescence cell counters of the LUNA™ family.\u003cbr\u003e\u003cbr\u003eAcridine orange permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red. Propidium iodide binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCompatible Instruments\u003c\/strong\u003e\u003cbr\u003eLUNA-FL\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003e™\u003c\/span\u003e Automated Cell Counter \u003cbr\u003eLUNA-STEM\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003e™\u003c\/span\u003e Automated Cell Counter \u003cbr\u003eLUNA-II YF\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003e™\u003c\/span\u003e Automated Yeast Cell Counter\u003cbr\u003eLUNA-FX7\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003e™\u003c\/span\u003e Automated Cell Counter\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProduct Description\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%; height: 137.157px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 30.1757%; height: 19.5938px;\"\u003eAppearance\u003c\/td\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003eOrange-red liquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 30.1757%; height: 39.1876px;\" rowspan=\"2\"\u003eCell permeability\u003c\/td\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003eMembrane permeable (acridine orange)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003eMembrane impermeant (propidium iodide)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 30.1757%; height: 39.1876px;\" rowspan=\"2\"\u003eExcitation\/emission\u003c\/td\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003e500\/526 nm (acridine orange)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003e533\/617 nm (propidium iodide)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 30.1757%; height: 19.5938px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003e4 °C in the dark\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eOrdering Information\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003ctd style=\"width: 30.1757%; height: 19.5938px;\"\u003eQuantity\u003c\/td\u003e\n\u003ctd style=\"width: 66.9671%; height: 19.5938px;\"\u003e2 x 0.5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.\u003cbr\u003eAdditional information is available on our website at\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.logosbio.com\/\" target=\"_blank\"\u003ewww.logosbio.com\u003c\/a\u003e\u003c\/p\u003e","brand":"logosbio","offers":[{"title":"Default Title","offer_id":48401127604446,"sku":"F23012","price":228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0651\/9393\/5070\/products\/LogosBiosystems-AOPI-Stain.png?v=1657955039","url":"https:\/\/shop.logosbio.com\/products\/acridine-orange-propidium-iodide-stain-5-x-0-1ml","provider":"logosbio","version":"1.0","type":"link"}