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Acridine Orange / Propidium Iodide Stain

Acridine Orange / Propidium Iodide Stain

F23001
Regular price $189.00 USD
Regular price Sale price $189.00 USD
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Acridine Orange/Propidium Iodide Stain is a cell viability dye that causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. Acridine Orange/Propidium Iodide Stain can be used to assess cell viability with the automated fluorescence cell counters of the LUNA™ family.

Acridine orange permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red. Propidium iodide binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.


Compatible Instruments
LUNA-FL Automated Cell Counter
LUNA-STEM Automated Cell Counter
LUNA-II YF Automated Yeast Cell Counter
LUNA-FX7 Automated Cell Counter



Product Description

Appearance Orange-red liquid
Cell permeability Membrane permeable (acridine orange)
Membrane impermeant (propidium iodide)
Excitation/emission 500/526nm (acridine orange)
533/617nm (propidium iodide)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark


This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com

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Description

Acridine Orange/Propidium Iodide Stain is a cell viability dye that causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. Acridine Orange/Propidium Iodide Stain can be used to assess cell viability with the automated fluorescence cell counters of the LUNA™ family.

Acridine orange permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red. Propidium iodide binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.


Compatible Instruments
LUNA-FL Automated Cell Counter
LUNA-STEM Automated Cell Counter
LUNA-II YF Automated Yeast Cell Counter
LUNA-FX7 Automated Cell Counter



Product Description

Appearance Orange-red liquid
Cell permeability Membrane permeable (acridine orange)
Membrane impermeant (propidium iodide)
Excitation/emission 500/526nm (acridine orange)
533/617nm (propidium iodide)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark


This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com