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Acridine Orange Stain

Acridine Orange Stain

F23002
Regular price $176.00 USD
Regular price Sale price $176.00 USD
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Acridine Orange Stain is a cell-permeant vital dye that binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red.

Acridine Orange Stain can be used with Propidium Iodide Stain (F23003) to assess cell viability with the automated fluorescence cell counters of the LUNA™ family. Viable nucleated cells will fluoresce green and nonviable nucleated cells will fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.


Compatible Instruments
LUNA-FL Automated Cell Counter
LUNA-STEM Automated Cell Counter
LUNA-II YF Automated Yeast Cell Counter
LUNA-FX7 Automated Cell Counter



Product Description

Molecular formula C17H20ClN3
Molecular weight 301.82 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 500/526 nm (when bound to DNA)
460/650nm (when bound to ssDNA or RNA)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark


This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com

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Description

Acridine Orange Stain is a cell-permeant vital dye that binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red.

Acridine Orange Stain can be used with Propidium Iodide Stain (F23003) to assess cell viability with the automated fluorescence cell counters of the LUNA™ family. Viable nucleated cells will fluoresce green and nonviable nucleated cells will fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.


Compatible Instruments
LUNA-FL Automated Cell Counter
LUNA-STEM Automated Cell Counter
LUNA-II YF Automated Yeast Cell Counter
LUNA-FX7 Automated Cell Counter



Product Description

Molecular formula C17H20ClN3
Molecular weight 301.82 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 500/526 nm (when bound to DNA)
460/650nm (when bound to ssDNA or RNA)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark


This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com