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DeepLabel™ Antibody Staining Kit

DeepLabel™ Antibody Staining Kit

C33001
Regular price $1,590.00 USD
Regular price Sale price $1,590.00 USD
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X-CLARITY™ Mounting Solution is a refractive index matching solution (RIMS) modified from the recipe developed by Yang et al (2). The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and fluorescence-labeled tissue samples.


Compatible Instruments
LUNA-II Automated Cell Counter
LUNA-FL Automated Cell Counter (for brightfield cell counting)
LUNA-FX7 Automated Cell Counter (for brightfield cell counting)
CELENA® S Digital Imaging System

 

DeepLabel™ Antibody Staining Kit is a set of reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding.

DeepLabel™ Solution A is a permeabilization reagent that enhances antibody permeation.

DeepLabel™ Solution B is an antibody dilution buffer that facilitates the antigen-antibody binding reaction. Unbound antibodies are efficiently removed by the DeepLabel™ Washing Buffer.

X-CLARITY™ Mounting Solution is a refractive index matching solution (RIMS) that minimizes photobleaching and preserves fluorescence signals. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range.

 


References
Lee, E. et al. ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3 dimensional (3D) imaging. Sci Rep 6, 18631 (2016)
Yang, B. et al. Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell 158, 945–958 (2014)
Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332–337 (2013)
Tainaka, K. et al. Whole-body imaging with single-cell resolution by tissue decolorization. Cell 159, 911-924 (2014)
Renier, N. et al. iDISCO: A simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896-910 (2014)



DeepLabel™ Antibody Staining Kit

DeepLabel™ Solution A 1 x 25 mL
DeepLabel™ Solution B 2 x 25 mL
DeepLabel™ Washing Buffer 1 x 250 mL
X-CLARITY™ Mounting Solution 1 x 25 mL

 

This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices. Additional information is available on our website at www.logosbio.com

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Description

X-CLARITY™ Mounting Solution is a refractive index matching solution (RIMS) modified from the recipe developed by Yang et al (2). The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and fluorescence-labeled tissue samples.


Compatible Instruments
LUNA-II Automated Cell Counter
LUNA-FL Automated Cell Counter (for brightfield cell counting)
LUNA-FX7 Automated Cell Counter (for brightfield cell counting)
CELENA® S Digital Imaging System

 

DeepLabel™ Antibody Staining Kit is a set of reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding.

DeepLabel™ Solution A is a permeabilization reagent that enhances antibody permeation.

DeepLabel™ Solution B is an antibody dilution buffer that facilitates the antigen-antibody binding reaction. Unbound antibodies are efficiently removed by the DeepLabel™ Washing Buffer.

X-CLARITY™ Mounting Solution is a refractive index matching solution (RIMS) that minimizes photobleaching and preserves fluorescence signals. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range.

 


References
Lee, E. et al. ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3 dimensional (3D) imaging. Sci Rep 6, 18631 (2016)
Yang, B. et al. Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell 158, 945–958 (2014)
Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332–337 (2013)
Tainaka, K. et al. Whole-body imaging with single-cell resolution by tissue decolorization. Cell 159, 911-924 (2014)
Renier, N. et al. iDISCO: A simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896-910 (2014)



DeepLabel™ Antibody Staining Kit

DeepLabel™ Solution A 1 x 25 mL
DeepLabel™ Solution B 2 x 25 mL
DeepLabel™ Washing Buffer 1 x 250 mL
X-CLARITY™ Mounting Solution 1 x 25 mL

 

This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices. Additional information is available on our website at www.logosbio.com