Propidium Iodide Stain
Propidium Iodide Stain
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Propidium iodide is a cell-permeant stain that binds to nucleic acids. Nonviable cells and cells with damaged membranes are the only ones that can take up propidium iodide, since intact membranes cannot penetrate it. Once bound to nucleic acids, its fluorescence is multiplied by 20-30, causing the cell to fluoresce red.
The LUNA™ Automated Fluorescent Cell Counter can use propidium iodide stain (F23003) with acridine orange stain (F23002)to assess cell viability. Viable nucleated cells with fluoresce green and non-viable cells will fluoresce red. Due to Förster resonance energy Transfer (FRET), the signal from propidium iodide absorbs the signal from acridine orange in non-viable cells, ensuring that double-positive results do not occur.
Compatible Instruments
LUNA-FL™ Automated Cell Counter
LUNA-STEM™ Automated Cell Counter
LUNA-II YF™ Automated Yeast Cell Counter
LUNA-FX7™ Automated Cell Counter
Product Description
| Molecular formula | C27H34I2N4 |
| Molecular weight | 668.39 g/mol |
| Appearance | Clear liquid |
| Cell permeability | Membrane permeant |
| Excitation/emission | 493/636 nm (in aqueous solution) |
| 533/617 nm (when bound to nucleic acids) | |
| Quantity | 2 x 0.5 mL |
| Storage | 4 °C in the dark |
This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com
Description
Propidium iodide is a cell-permeant stain that binds to nucleic acids. Nonviable cells and cells with damaged membranes are the only ones that can take up propidium iodide, since intact membranes cannot penetrate it. Once bound to nucleic acids, its fluorescence is multiplied by 20-30, causing the cell to fluoresce red.
The LUNA™ Automated Fluorescent Cell Counter can use propidium iodide stain (F23003) with acridine orange stain (F23002)to assess cell viability. Viable nucleated cells with fluoresce green and non-viable cells will fluoresce red. Due to Förster resonance energy Transfer (FRET), the signal from propidium iodide absorbs the signal from acridine orange in non-viable cells, ensuring that double-positive results do not occur.
Compatible Instruments
LUNA-FL™ Automated Cell Counter
LUNA-STEM™ Automated Cell Counter
LUNA-II YF™ Automated Yeast Cell Counter
LUNA-FX7™ Automated Cell Counter
Product Description
| Molecular formula | C27H34I2N4 |
| Molecular weight | 668.39 g/mol |
| Appearance | Clear liquid |
| Cell permeability | Membrane permeant |
| Excitation/emission | 493/636 nm (in aqueous solution) |
| 533/617 nm (when bound to nucleic acids) | |
| Quantity | 2 x 0.5 mL |
| Storage | 4 °C in the dark |
This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com